Journal: Vaccines
Article Title: Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines
doi: 10.3390/vaccines11050937
Figure Lengend Snippet: Intra-laboratory repeatability of spiked samples with different concentration levels (50%, 75%, 100%, 125%, 150%, 200%) from each laboratory: ( a ) DMG-PEG2K; ( b ) CHOLE; ( c ) DSCP; ( d ) 9001.
Article Snippet: Methanol (liquid chromatograph ion spray mass spectroscopy/LCMS-grade), ethyl alcohol (LCMS-grade), and triethylamine acetate (high-performance liquid chromatography/HPLC-grade) were purchased from Thermo Fisher Scientific (Waltham, MA, USA); 1,2-dimyristoyl-rac-glycero-3-methoxypolyethylene glycol-2000 (DMG-PEG2K) was purchased from NOF America Corporation (Irvine, CA, USA); 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) (>99%) and cholesterol were purchased from Nippon Fine Chemical (Osaka, Japan); and 9001 was synthesized by Abogen (Suzhou, China).
Techniques: Concentration Assay